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KMID : 0613820080180121679
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Journal of Life Science
2008 Volume.18 No. 12 p.1679 ~ p.1685
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Conjugated Linoleic Acid Induces Apoptosis by Activating AMPK in MCF-7 Breast Cancer Cells
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Lin Sun-Kyo
Kim Hyun-Sook
Park Ock-Jin
Kim Young-Min
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Abstract
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Conjugated linoleic acid (CLA) is a naturally occurring compound found in dairy and beef products. It has been shown to suppress cancer cells and to induce apoptosis. Practically, there is emerging evidence that CLA can inhibit chemically induced carcinogenesis in various tissues. However, the molecular mechanisms of CLA on human MCF-7 breast cancer cells have not been clearly explained yet. In this report, we investigated the anti-cancer activity of CLA in MCF-7 cells. It was found that CLA could inhibit the growth of the MCF-7 cells and induce apoptosis, through modulating AMP-activated protein kinase (AMPK) and cyclooxygenase-2 (COX-2). AMPK acts as a cellular fuel gauge and responds to decreased cellular energy status by inhibiting ATP-consuming pathways and increasing ATP-synthesis. CLA treatment with variable concentrations and different time of same-dose CLA on MCF-7 cells resulted in a strong activation of AMPK and an inhibition of COX-2 expression. It supports that CLA induces apoptosis in CLA-treated MCF 7 cells. Therefore, the effects of CLA induced COX-2 expression via activating AMPK can provide new possibility into the understanding the molecular mechanisms of anti-cancer component.
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KEYWORD
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Conjugated linoleic acid, MCF-7 cells, apoptosis, AMP-activated protein kinase, cyclooxygenase-2
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